Dr. Osada, Kensuke
Presentation day
Thursday 7:40 PM
TITLE
Tadpole-shaped polymeric micelle with distinct phase segregation formed from pDNA, block copolymers, and chondroitin sulfate
Abstract
Supramolecular self-assembly has emerged as a worthy tool in fabrication of
nanostructures to pursue desired structures and functionalities particularly for demand of nanotechnology in
medical applications. In this report, we would like to present an unprecedentedly found novel nanostructure
of polymeric micelle, characterized as distinct tadpole-shaped structure, possessing spherical head and
extended shaft in single plasmid DNA (pDNA). The obtained tadpole-shaped structure was constructed by
adding anionic chondroitin sulfate (CS) to a rod-shaped polyplex micelle where single pDNA
macromolecule was packaged by PEG-polycation block copolymer into regularly folded DNA bundle
through their electrostatic self-assembly. Subsequent investigations revealed that CS could interact with
polyplex micelle to develop a ternary system and the CS distribution preferentially localized in one end to
form head of tadpole, presumably as a consequence of immiscibility of CS-rich phase and CS-poor phase.
Eventually, substantial osmotic pressure could be developed at this CS accumulated rod to form swollen
head. It should be addressed that the tadpole-shaped system demonstrated not only reducing in vitro
cytotoxicity, in vivo tissue damage and inflammatory response, but also high in vivo efficiency for
intratracheal gene transfection, thus possessing an advantage utility as a safe and efficient gene delivery
carriers.
CV
2002 Ph.D., Dept. of Organic and Polymeric Materials, Tokyo Institute of Technology（JSPS DC1 fellowship）
2002- Postdoctoral fellow of JSPS: Dept. of Materials Engineering, The University of Tokyo
2004- Assistant Professor: Dept. of Materials Engineering, The University of Tokyo
2006- Associate Professor: Dept. of Materials Engineering, The University of Tokyo
2012- JST-PRESTO Researcher
2013- Associate Professor: Dept. of Bioengineering, The University of Tokyo
Literatures
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