Dr. Kameta, Naohiro
Presentation day
Thursday 7:00 PM
TITLE
Soft Nanotubes for Encapsulation, Release, and Stabilization of Proteins
Abstract
Self-assembly of rationally designed amphiphiles in water selectively gave nanotubes with
a well-defined size dimension and functional surfaces. The nanotube channels with 5-100 nm inner
diameters shaped by monolayer lipid membranes of the amphiphiles were able to act as meso-scale hosts
to encapsulate biomacromolecules such as DNAs and proteins. Switching from storage to release for the
encapsulated guests was precisely controllable by external stimuli such as pH and temperature. Time-lapse
fluorescence microscopy combined with fluorescence resonance energy transfer system enabled us to
visualize the transportation of proteins in the nanotube channel. The diffusion coefficient of the protein in
the nanotube channel was remarkably smaller than that of free protein in bulk solutions. The confined
proteins in the nanotube channel kept the biological activity under harsh conditions of high temperatures
and high concentrations of denaturants. Furthermore, the nanotubes were also able to function as artificial
chaperones, and assist the refolding of chemically-denatured proteins. Hydrophobic interactions between
the nanotube channels that were modified with hydrophobic groups and the surface-exposed hydrophobic
amino acid residues of the denatured proteins in the intermediately refolded state remarkably enhanced the
refolding efficiency. Size balance between the nanotube channel and the proteins were also important for
the refolding efficiency. Such biofunctions of the nanotubes will open ways to develop drug delivery
systems, enzyme reactors, protein drugs, and so on.
References (Review articles): 1) N. Kameta, J. Incl. Phenom. Macrocycle. Chem. 79, 1 (2014). 2) T.
Shimizu, H. Minamikawa, M. Kogiso, M. Aoyagi, N. Kameta, W. Ding, M. Masuda, Polym. J. 46, 831
(2014).
Institution
National Institute of Advanced Industrial Science and Technology (AIST) ,
Tsukuba, Ibaraki, Japan
Literatures
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Kameta, N., Masuda, M. & Shimizu, T. (2012) Soft nanotube hydrogels functioning as artificial chaperones, ACS Nano, 6, 5249-5258.
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Kameta, N., Ishikawa, K., Masuda, M. Asakawa, M. & Shimizu, T. (2012) Soft nanotubes acting as a light-harvesting antenna, Chem. Mater., 24, 209-214.
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Kameta, N., Ishikawa, K., Masuda, M. & Shimizu, T. (2013) Control of self-assembled morphology and molecular packing of asymmetric glycolipids by association/dissociation with poly(thiopheneboronic acid), Langmuir, 29, 13291-13298.
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Kameta, N., Lee, S. J., Masuda, M. & Shimizu, T. (2013) Biologically responsive, sustainable release from metallo-drug coordinated 1D nanostructures, J. Mater. Chem. B, 1, 276-283.
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Ishikawa, K., Kameta, N., Aoyagi, M. Asakawa, M. & Shimizu, T. (2013) Soft nanotubes with a hydrophobic channel hybridized with Au nanoparticles: photothermal dispersion / aggregation control of C60 in water , Adv. Funct. Mater., 23, 1677-1683.
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Ishikawa, K., Kameta, N., Masuda, M. Asakawa, M. & Shimizu, T. (2014) Boroxine nanotubes: moisture-sensitive morphological transformation and guest release, Adv. Funct. Mater., 24, 603-609.
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Kameta, N. (2014) Soft nanotube hosts for capsulation and release of molecules, macromolecules, and nanomaterials, J. Incl. Phenom. Macrocycle. Chem, 79, 1-22.
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Kameta, N., Masuda, M. & Shimizu, T. (2015) Photoinduced morphological transformations of soft nanotubes, Chem. Eur. J., 21, 8832-8839.
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Kameta, N., Masuda, M. & Shimizu, T. (2015) Two-step naked-eye detection of lectin by hierarchical organization of soft nanotubes into liquid crystal and gel phases, Chem. Commun., 51, 6816-6819.
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Kameta, N., Masuda, M. & Shimizu, T. (2015) Qualitative / chiral sensing of amino acids by naked-eye fluorescence change based on morphological transformation and hierarchizing in supramolecular assemblies of pyrene-conjugated glycolipids, Chem. Commun., 51, 11104-11107.
CV
2002: Ph.D. (Analytical chemistry), Ibaraki University.
2002-2003: Post doc. (Supramolecular chemistry), Utsunomiya University.
2004-2005: Post doc. (Nanostructure chemistry), National Institute of Advanced Industrial Science and Technology (AIST).
2006-2007: Post doc. (Nanospace chemistry) CREST-SORST, Japan Science and Technology Agency (JST).
2008-2012: Researcher (Organic nanomaterials), AIST.
2011 (3 months): Visiting researcher, Institut Européen de Chimie et Biologie (IECB-CNRS, France) and National Institute for Nanotechnology (NINT, Canada)
2013- : Senior Researcher (Functional surface materials), AIST.