Dr. Edler, Karen
Institution
Department of Chemistry, University of Bath, Bath, United Kingdom.
Presentation day
Wednesday 8:20 AM
TITLE
Self-assembly of lipid-polymer nanodiscs for membrane protein supports
Abstract
Phospholipid bilayer nanodiscs are novel self-assembled model membrane structures
composed of a bilayer of phospholipids with the hydrophobic disc edges surrounded and stabilized by a
polymer1,2 or protein3 belt. They can be used to solubilise membrane proteins, allowing structural and
functional studies of this class of proteins, which are otherwise difficult to stabilize outside of the native cell
membrane. Polymer stabilized nanodiscs are made directly from cell membranes simply by adding polymer
and shaking, without requiring addition of detergent or ultrasound to assist dispersion so are useful for
membrane protein purification & separation.1,2 Nanodiscs can also encapsulate hydrophobic species for
potential applications in drug delivery. We are working to understand the properties of nanodiscs stabilized
by acid and amine derivatives of poly(styrene-co-maleic anhydride) using small angle neutron and X-ray
scattering (SANS/SAXS), DLS, cryo-TEM and by variation of the polymer structure. These polymer
stabilizers can be made in bulk quantities, more cheaply than the protein belt, enabling large quantities of
discs to be prepared. In this presentation I will discuss the effects of polymer chemistry and structure on
disc formation, the effect of different lipids and initial structural studies of membrane proteins supported in
polymer-lipid nanodiscs. The use of molecular modelling to fit SANS/SAXS data from nanodisc systems
using the SASSIE program suite will also be discussed.
CV
Prof Karen Edler is Professor of Soft Matter in Physical Chemistry at the University of Bath, and is also currently Visiting Professorship in Physical Chemistry at Lund University, Sweden. She did her BSc/LLB at Sydney University and her PhD in the Research School of Chemistry at ANU, with Prof JW White, followed by a postdoc at Cornell University with Prof S Gruner (Physics) on L3-phase templated silicas. She moved to Bath in 1999 to take up a joint PDRA position at Bristol and Bath, working for Prof S Mann & Dr S Roser on the self-assembly of surfactant templated silica films. She was awarded a Dorothy Hodgkin Royal Society Research Fellowship in 2000, resulting in her appointment at Bath, where she remains. Her research lies in the area of self-assembly in soft matter, biomimetic materials, polymer-surfactant self-organisation and also intersects with sol-gel oxides and porous inorganic materials. Most of her research involves use of a range of X-ray and neutron scattering techniques to probe nanostructures and formation mechanisms in solution and at interfaces in these systems.
Literatures
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Dongmei Jiang, Andrew D. Burrows, Yuli Xiong, and Karen J. Edler, J. Mater. Chem. A. 1, 5497-5500(2013) DOI:10.1039/C3TA10766C. Facile synthesis of crack-free metal-organic framework films on alumina by a dip-coating route in the presence of polyethylenimine
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Aurora Perez-Gramatges, Hansel Comas-Rojas, Carlos Enriquez-Victorero, Karen J. Edler , Stephen J. Roser, Soft Matter 9, 4003-4014 (2013) DOI:10.1039/C3SM27541H. Self-assembly and phase behavior of PEI:cationic surfactant aqueous mixtures forming mesostructured films at the air/solution interface
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Elenica Shiko, Karen J. Edler, John P. Lowe and Sean P. Rigby, Journal of Colloid & Interface Science398, 168-175 (2013) DOI:10.1016/j.jcis.2013.02.002. Probing hysteresis during sorption of cyclohexane within mesoporous silica using NMR cryoporometry and relaxometry
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Bin Yang, James A. Holdaway and Karen J. Edler, Langmuir 29, 4148 - 4158 (2013) DOI:10.1021/la4001329. Robust Ordered Cubic Mesostructured Polymer/Silica Composite Films Grown at the Air/Water Interface
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Karen J Edler and Bin Yang, Chem. Soc. Rev. 42, 3765 - 3776 (2013) DOI:10.1039/C2CS35300H. Formation of mesostructured thin films at the air-liquid interface
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Dongmei Jiang, Luke L. Keenan, Andrew D. Burrows and Karen J. Edler, Chem Commun. 48, 12053-12055 (2012) DOI:10.1039/c2cc36344e. Synthesis and post-synthetic modification of MIL-101(Cr)-NH2via a tandem diazotisation process
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Sheng Qi, Steve Roser , Karen J. Edler, Claudia Pigliacelli, Madeleine Rogerson, Ilse Weuts, Frederic Dycke, Sigrid Stokbroekx, Pharm Res, 30, 290 - 302 (2012) DOI: 10.1007/s11095-012-0873-7. Insights into the role of polymer-surfactant complexes in drug solubilisation/stabilisation during drug release of solid dispersions
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Dongmei Jiang, Andrew D. Burrows, Karen J Edler, CrystEngComm. 13, 6916 - 6919 (2011), DOI: 10.1039/c1ce06274c. Size-controlled synthesis of MIL-101(Cr) nanoparticles with enhanced selectivity for CO2 over N2