Dr. Dohno, Chikara
Institution
The institute of Scientific and Industrial Research, Osaka University, Ibaraki,Osaka, Japan.
Presentation day
poster
TITLE
Modulation of binding properties of DNA assemblies to lipid bilayer membrane
Abstract
DNA is a promising molecular unit for constructing well-defined functional structures.
Recent development of DNA nanotechnology enables us to construct almost arbitrary 2D and 3D
nanometer-scale structures. Lipid membranes have emerged as an attractive target for the DNA
nanostructures, which may design diverse functions as membrane-associating proteins do. It is important to
know binding properties of such DNA nanostructures with a wide variety of 2D, 3D-shapes to lipid
membranes and how the binding of the DNA assemblies affects biophysical properties of lipid membranes.
We recently have synthesized amphiphilic DNAs having hydrophobic moieties which can bind effectively to
lipid bilayer membrane. We here introduced the amphiphilic DNAs into 2D DNA nanostructures constructed
with DNA tile and DNA origami methods. AFM measurements clearly showed that the DNA assemblies
bound to surface of lipid bilayer membranes. Presence of divalent cations promoted the binding of DNA
assemblies to lipid membranes even in the absence of hydrophobic modifications. In the conditions of low
concentration of divalent cations, the hydrophobic modification is important for binding of the DNA
assemblies. The binding was dependent on modification sites and number of the hydrophobic modifications
in the DNA assemblies, shape of the assemblies and composition of lipid molecules.
CV
997 B.S., Department of Industrial Chemistry, Faculty of Engineering, Kyoto University
1999 M.S., Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University
2002 Ph.D., Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University (Prof. Isao Saito)
2002-2003 Postdoctoral fellow, Department of Chemistry, California Institute of Technology (Prof. Jacqueline K. Barton)
2003-2005 Postdoctoral fellow, Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University (Prof. Isao Saito),
and Department of Chemistry, Graduate School of Science, Kyoto University (Prof. Hiroshi Sugiyama)
2005-2011 Assistant professor, The Institute of Scientific and Industrial Research, Osaka University (Prof. Kazuhiko Nakatani)
2010.10-2014.3 PRESTO Researcher, Japan Science and Technology Agency (concurrent post)
2011.3-present Associate professor, The Institute of Scientific and Industrial Research, Osaka University (Prof. Kazuhiko Nakatani)
Literatures
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A hybridisation-dependent membrane-insertable amphiphilic DNA,Dohno, C.; Matsuzaki, K.; Yamaguchi, H.; Shibata, T.; Nakatani, K. Org. Biomol. Chem. 2015, 13, 10117-10121.
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Modulation of binding properties of amphiphilic DNA containing multiple dodecyl phosphotriester linkages to lipid bilayer membrane,Makishi, S.; Shibata, T.; Okazaki, M.; Dohno, C.; Nakatani, K. Bioorg. Med. Chem. Lett., 2014, 24, 3578-3581.
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Formation of Ligand-Assisted Complex of Two RNA Hairpin Loops,Hong, C.; Otabe, T.; Matsumoto, S.; Dohno, C.; Murata, A.; Hagihara, M.; Nakatani, K. Chem. Eur. J., 2014, 20, 5244-5252.
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A synthetic riboswitch that operates using a rationally designed ligand-RNA pair.
Dohno, C.; Kohyama, I.; Kimura, M.; Hagihara, M.; Nakatani, K. Angew. Chem. Int. Ed. 2013, 52, 9976-9979.
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G-quadruplex formation of entirely hydrophobic DNA in organic solvents.
Shibata, T.; Dohno, C.; Nakatani, K. Chem. Commun. 2013, 49, 5501-5503.
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Ligand-induced electron spin-assembly on a DNA tile.
Atsumi, H.; Nakazawa, S.; Dohno, C.; Sato, K.; Takui, T.; Nakatani, K. Chem. Commun. 2013, 49, 6355-6458.
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Amphiphilic DNA duplex stabilized by hydrophobic zipper.
Dohno, C.; Shibata, T.; Okazaki, M.; Makishi, S.; Nakatani, K. Eur. J. Org. Chem. 2012, 5317-5323.
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Naphthyridine tetramer with a preorganized structure for 1:1 binding to a CGG/CGG sequence.
Dohno, C.; Kohyama, I.; Hong, C.; Nakatani, K. Nucleic. Acids. Res. 2012, 40, 2771-2781.
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Control of DNA hybridization by photoswitchable molecular glue,
Dohno, C.; Nakatani, K. Chem. Soc. Rev. 2011, 40, 5718-5729.
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Molecular-Glue-Triggered DNA Assembly to Form a Robust and Photoresponsive Nano-Network,
Wang, C.; Pu, F.; Lin, Y.; Ren, J.; Dohno, C.; Nakatani K.; Qu, X. Chem. Eur. J. 2011, 17, 8189-8197.
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Ligand Inducible Assembly of a DNA Tetrahedron.
Dohno, C.; Atsumi, H.; Nakatani, K. Chem. Commun. 2011, 47, 3499-3501.
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A Light-Driven, Supramolecular Optical Switch,Uno, S.; Dohno, C.; Bittermann, H.; Malinovskii, V. L.; Häner, R.; Nakatani, K. Angew. Chem. Int. Ed. 2009, 48, 7362-7365.